5-Amino-1MQ

**5-Amino-1-methylquinolinium (5-Amino-1MQ)** 5-Amino-1-methylquinolinium, commonly referred to as 5-Amino-1MQ, is a small molecule methylquinolinium derivative that functions as a selective, membrane-permeable inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme primarily expressed in the liver and adipose tissue, where it plays a critical role in cellular energy homeostasis by catalyzing the methylation of nicotinamide (NAM) using S-adenosyl-L-methionine (SAM) as the methyl donor. In laboratory research frameworks, 5-Amino-1MQ is utilized to investigate the modulation of the NAD+ salvage pathway. By inhibiting NNMT-mediated depletion of nicotinamide, this compound facilitates an increase in intracellular NAD+ levels and a subsequent shift in cellular metabolic flux, making it a primary tool for studying sirtuin activity and mitochondrial biogenesis in vitro. In preclinical research settings, 5-Amino-1MQ is frequently employed to explore the metabolic intersections of obesity, type 2 diabetes, and muscle atrophy models. Research indicates that inhibiting NNMT can influence the expression of genes involved in gluconeogenesis and lipid metabolism without interacting with other methyltransferases or nicotinamide phosphoribosyltransferase (NAMPT). This high degree of selectivity allows researchers to isolate the effects of NAM/NAD+ ratios on cellular signaling, specifically the activation of Adenosine Monophosphate-activated Protein Kinase (AMPK). In cell culture models, researchers observe that 5-Amino-1MQ may counteract the adipocyte hypertrophy and metabolic dysfunction associated with high-fat substrate environments, providing a robust platform for investigating the pharmacological regulation of metabolic rate at the molecular level. For laboratory application, 5-Amino-1MQ is typically provided as a stable powder. It is soluble in organic solvents such as DMSO or ethanol; however, researchers should ensure complete dissolution before proceeding with serial dilutions in aqueous buffers to prevent precipitation. For long-term preservation of biological activity, the compound should be stored at -20°C in an airtight container, protected from direct light and moisture. Once reconstituted in a solvent, it is recommended to use the solution immediately or aliquot and store at -80°C to avoid repeated freeze-thaw cycles. **This product is intended for IN-VITRO RESEARCH AND LABORATORY USE ONLY. It is not for human consumption, clinical use, or veterinary applications.**